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FIGURE 5. Dominant negative dynamin inhibits membrane drift into spines. a, sample of images of the dendritic shaft of a neuron expressing mCherry-tagged dynamin 2K44A and mGFP presented as an inverted color map for monochromatic images. Scale bar = 1 µm. b, scatter plots of computed diffusion coefficients of mGFP in spines and dendritic shafts. The median values in µm2/s are: on spines, control (mGFP alone), n = 20, 0.302; Dyn2WT, n = 18, 0.475; Dyn2K44A, n = 21, 0.070, p < 0.01 versus Dyn2WT); on dendritic shaft, control (mGFP alone), n = 5, 1.68; Dyn2WT, n = 5, 1.47; Dyn2K44A, n = 5, 1.17, ns). c, mean ± S.E. of mobile fraction of mGFP in spines (control (mGFP alone), n = 20, 0.79 ± 0.03; Dyn2WT, n = 24, 0.64 ± 0.06; Dyn2K44A, n = 21, 0.65 ± 0.03, p < 0.05 versus control) and on the dendritic shaft (control (mGFP alone), n = 5, 0.65 ± 0.06; Dyn2WT, n = 5, 0.62 ± 0.05; Dyn2K44A, n = 5, 0.65 ± 0.04). d, normalized mGFP FLIP traces recorded in Dyn2WT- and Dyn2K44A-transfected neurons from the FLIP region (black), the shaft (gray), and the spine (red). Thin traces are corresponding fits, and arrows indicate the time constant computed for the exponential decay fit (Dyn2WT, shaft, 6.45 s, spine, 4.45 s; Dyn2K44A, shaft, 4.29 s, spine, 12.41 s). e, mean ± S.E. of time constant ratio (spine/shaft) measured during FLIP experiments (control (mGFP alone), 1.49 ± 0.16; Dyn2WT, 1.42 ± 0.28; Dyn2K44A, 2.98 ± 0.30; Dyn2WT versus Dyn2K44A, p < 0.05).

This figure was published in "Dynamin-dependent Membrane Drift Recruits AMPA Receptors to Dendritic Spines", Frédéric Jaskolski, Belen Mayo-Martin, David Jane and Jeremy M. Henley, J Biol Chem. 2009 May 1; 284(18): 12491–12503. doi: 10.1074/jbc.M808401200.